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Sample text

Some insects keep the spiracles hermetically closed most of the time. They are able to actively regulate the passage of air through some selected spiracles. These programmed inspirations and expirations of air, as well as the decision which spiracle will open at what time is also under control of the coelopulse system (SHima, 1988). J 0 36 «z ~ 0 0 en « 12, . ) 15 18 Figure 4. Pulsations in a normal diapausing females of B. affinis (above) and a few hrs following decapitation (below), 20°C. monitoring the respiratory scenarios of diapausing B.

The solutions needed for any desired relative humidity were put into the desiccators at least a day before starting the experiment and the solutions were also renewed weekly to assure desired humidities. Counting the eggs and the methods of differentiating various insect instars will be illustrated in their proper places in the text. Temperatures and relative humidities were recorded daily. Under controlled conditions four constant temperatures were used viz. 20, 24, 28 and 32°C. and four relative humidities viz.

20, 24, 28 and 32°C. and four relative humidities viz. 20, 40, 60 and 80%. Adult insects were taken from stock cultures of infested broad beans and the seeds in which new eggs were deposited were collected directly and kept in glass tubes (lx2 inches) covered with muslin and incubated. Newly hatched larvae were isolated in separate tubes covered with muslin and fixed tightly with rubber bands. Fifty pairs of male and female adults were used in each experiment. All observations were recorded daily and as was the duration of the different developmental stages.

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