Download PCR Technology: Current Innovations, Third Edition by Tania Nolan, Stephen A. Bustin PDF

By Tania Nolan, Stephen A. Bustin

PCR’s simplicity as a molecular procedure is, in many ways, accountable for the large quantity of innovation that surrounds it, as researchers regularly ponder new how one can tweak, adapt, and re-formulate ideas and functions. PCR Technology: Current recommendations, 3rd variation is a suite of novel equipment, insights, and issues of view that gives a severe and well timed reference element for a person wishing to take advantage of this technology.

Topics during this forward-thinking quantity include:

  • The purification and dealing with of PCR templates
  • The impression of the manufacture and purification of the oligonucleotide on PCR behavior
  • Optimum buffer composition
  • Probe options
  • The layout and optimization of qPCR assays
  • Issues surrounding the advance and refinement of instrumentation
  • Effective controls to guard opposed to uncertainties because of response variability

Covering all features of PCR and real-time PCR, the publication includes specific protocols that make it compatible as either a reference and an handbook. each one bankruptcy provides particular directions in addition to necessary tricks and assistance provided via authors who're well-known specialists of their fields. as well as descriptions of present know-how and top practices, the publication additionally presents information regarding new advancements within the PCR arena.

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Extra info for PCR Technology: Current Innovations, Third Edition

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Mechanisms underlying the impact of humic acids on DNA quantification by SYBR Green I and consequences for the analysis of soils and aquatic sediments. Nucleic Acids Res 31:e39. 11. , S. Fuchs, and M. Wagner. 2010. The fluorescence characteristics of enrichment media in the wavelength range of real-time PCR thermocycler optical path assignments. Food Anal Methods 3:219–224. 12. N. R. Siragusa. 1996. Evaluation of six sampling methods for recovery of bacteria from beef carcass surfaces. Lett Appl Microbiol 22:39–41.

Glencross, R. W. Griffiths. 2006. Direct quantitation and detection of salmonellae in biological samples without enrichment, using two-step filtration and real-time PCR. Appl Environ Microbiol 72:3896–3900. 54. , Y. Blixt, H. Grage, E. Borch, and P. Rådström. 2002. Evaluation of selective enrichment PCR procedures for Yersinia enterocolitica. Int J Food Microbiol 73:35–46. 55. , F. Tjerneld, B. Hahn-Hägerdal, and P. Rådström. 1996. Use of aqueous two-phase systems in sample preparation for polymerase chain reaction-based detection of microorganisms.

W. Griffiths. 2005. Microbiological sampling of poultry carcass portions by excision, rinsing, or swabbing. J Food Prot 68:2718–2720. 20. P. Buttner, and P. Cruz. 2004. Detection and enumeration of airborne biocontaminants. Curr Opin Biotechnol 15:170–174. 21. , P. D. Stetzenbach, and T. Cronin. 2007. Evaluation of two surface sampling methods for detection of Erwinia herbicola on a variety of materials by culture and quantitative PCR. Appl Environ Microbiol 73:3505–3510. J. Rose, A. Peterson, J.

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