By Alton C. Morgan Jr., Robert W. Schroff (auth.), Kenneth A. Foon M.D., Alton C. Morgan Jr. PH.D. (eds.)
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Extra resources for Monoclonal Antibody Therapy of Human Cancer
15. Natali PG ,Cavaliere R, Bigotti A, et a1. J Immuno1 130: 1462-1466. 16. Marx JL. 1981. Science 216:283-285. 17. Yeh M-Y, Hellstrom I, He11str5m KE. 1981. J Immuno1 126: 13121317. 18. Il, Morgan AC Jr. 1985. Int J Cancer, in press. 19. Nakapou1ou L, Zinozi M, Theodoropou1os G, Papachara1ampous N. 1982. Dis Colon Rectum 26:269-274. 20. He11str5m I, Carrigues HJ;-tabasco L, Mosely GH, Brown JP, Hellstrom KE. 1983. J Immuno1 130:1467-1472. 21. Burshie1 SW, Martin JC, Imai K~errone S, Warner NL.
I GO I/) .... It\ 1 79lT 1 cells PA-I cells 20 lrl 1~ Hi'- Vindeslne concentration ( ng I ml ) FIGURE 13. Effect of vindesine (VDS) and VDS-79lT/36 monoclonal antibody on survival of tumor cells in culture. Cells were treated with the agent for 15 min, then washed and cultured for 24 hr. They were then labeled for 16 hr wi th 7 Sse-met and washed 3 times. Uptake of 7SSe by treated cells is expressed as a percentage of that in controls (treated with phosphate-buffered saline). 79lT/36, osteogenic sarcoma.
Cell division so that the mean colony size showed minimal change I but the IC90 dose allowed colony growth to continue, albeit at a lower rate and incidence than in controls. These results show that the conjugate acted in a cytostatic rather than cytolytic fashion when the target cells were in a logarithmic growth phase. Cytostatic inhibition was most effective in single cells before division had occurred and was a relatively slow process in that many cells, exposed for 2 days to a partially toxic dose, could still recover in fresh growth medium.