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C. Srivastava, A. S. Raghubanski,J. S. Singh & S. P. Singh, 1991. Microbial C. Nand P in dry tropical Savannah: effects of burning and grazing. J. App!. 28: 869-878. , K. G. Boto. D. M. Alongi & F. T. Gillan, 1987. Composition and bacterial utilization of free amino acids in tropical mangrove sediments. Mar. Chem. 22: 1330. Tenore, K. , 1988. Nitrogen in benthic food chains. In: Blackburn, T. , Sorensen, J. (eds). Nitrogen Cycling in Coastal Marine Environments, Wiley & Sons, Chichester: 191-206.
Materials and methods Growth N. conspicua Grunow (isolate number 111) was isolated in axenic state (Hoshaw & Rowowski, 1973) from boat scrapings collected from Umbai, Melaka. The culture is deposited at the Algal Culture Collection at the Institute of Advanced Studies, University of Malaya. 64 x 10- 4 mM). The cultures were grown in conical flasks (cap. 21) and agitated by magnetic stirring to prevent cell clumping and settlement. LEm - 2 s - 1) with a 12: 12 h light-dark cycle in a controlled temperature room (26-28 0e).
Katoh & E. Sada, 1983. Arachidonic acid production by the red alga Porphyridium cruentum. Biotech. Bioeng. 25: 157-170. , T. G. Tornabene & W. H. Thomas, 1985. Chemical profile of selected species of microalgae with emphasis on lipids. J. Phycol. 21: 72-81. Bligh, E. G. & W. J. Dyer, 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physio\. 37: 911-917. Borowitzka, M. , 1988. Fats, oils and hydrocarbons. In M. A. Borowitzka & L. J. Borowitzka (eds), Microalgal Biotechnology.