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By Robert Ducluzeau, Marc Cerf, Gérard Corthier, Jean-Claude Rambaud, Paul Chapoy, Yannick Aujard, Gary W. Elmer (auth.), Professeur Eugénie Bergogne-Berezin (eds.)

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None of these associations have been clearly shown to be pathological. JEJUNAL SAMPLES a e Fig. l. a Bacterial counts from serial jejunal biopsies in patients with Billroth II gastrectomy . Biopsies were taken at I 0 em intervals and homogenized . Aliquots of homogenates were diluted from 10 - 1 to 10 - 6 and cultivated on various media . Results are expressed as logiO counts per g fresh tissue . coli associated with jejunal microvilli in a patient with Billroth II gastrectomy b 4 '/ •••••• ........

Although buccal cells have been shown to fix various bacteria including enterobacteria, studies on intestinal microflora demand other « physiological » models. Recent studies have focused on bacterial adhesion to isolated human intestinal and human fetal cells. Organotypic cultures have also been assayed. Colonic cells were used in a study on transformed non adherent bacteria in which either the pi/ region coding for type 1 fimbriae or the pap region encoding for assembly of P fimbriae had been inserted [83].

A heterogenous structure has been evidenced for another antigen (PCF 8775) with 3 components called C4, C5, C6. Whether other adhesins also have a complex composition is unknown. 5-1 llm long fine rods with an outer diameter of 7 nm fixed onto the bacterial surface. They are polymeric structures resulting from heliceal arrangements made of about 100 peptide subunits with a molecular weight from about 12,000 to 30,000 D. These peptide subunits include a high proportion of hydrophobic amino-acids.

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