Download Introduction to Modern Virology 6th Edition by Nigel Dimmock, Andrew Easton, Keith Leppard PDF

By Nigel Dimmock, Andrew Easton, Keith Leppard

Advent to trendy Virology has been a longtime scholar textual content for over 25 years. offering an built-in account of the topic throughout varied host platforms, with an emphasis on human and animal viruses, this booklet covers the sphere of virology from molecular biology to ailment strategies utilizing a distinct structures strategy. that includes an all new artwork software in complete colour, the recent version has been up-to-date all through, and reorganized into thematic sections at the primary nature of viruses, their progress in cells, their interactions with the host organism and their position as brokers of human ailment. there's a new bankruptcy on Human Viral disorder and speedily constructing components, equivalent to using viruses as gene remedy vectors, were included.The sixth version is much more obtainable, now together with key issues and integrative questions in each bankruptcy, in addition to textual content containers emphasizing take-home messages, facts underpinning the most ideas, and additional details for extra complex readers. Prevention and treatment, evolution and rising viruses obtain specific realization and particular chapters deal with the foremost infectious demanding situations posed via HIV, pandemic influenza and BSE. This hugely available textual content offers excellent analyzing for all undergraduate and postgraduate scholars of biology and medication wishing to check virology.Artwork from the booklet and useful scholar and teacher assets can be found on-line at www.blackwellpublishing.com/dimmock.An teacher handbook CD-ROM for this identify is obtainable. Please touch our better schooling staff at HigherEducation@wiley.com for additional information.

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Extra resources for Introduction to Modern Virology 6th Edition

Sample text

Diagnosis of influenza virus: coming to grips with the molecular era. Molecular Diagnosis 6, 291–305. Wiedbrauk, D. , Farkas, D. H. 1995. Molecular Methods for Virus Detection. Academic Press, New York. Wild, D. 2001. The Immunoassay Handbook, 2nd edn. Nature Publishing Group, London. Also check Appendix 7 for references specific to each family of viruses. 30 PART I WHAT IS A VIRUS? 3 The structure of virus particles All virus genomes are surrounded by proteins which: • Protect nucleic acids from nuclease degradation and shearing.

24 PART I WHAT IS A VIRUS? Slow rotation Level of culture medium Cells grow on the length of the helical plastic inserts (a) Fig. 5 (a) One way to increase cell density is by increasing the surface area to which cells can attach; this is a view from the end of a bottle lined with spiral plastic coils. The bottle is rotated slowly, at about 5 rev/h, so that a small volume of culture fluid can be used. Cells tolerate being out of the culture fluid for short periods. (b,c) Cells growing on microcarrier beads.

PCR has the added advantage of detecting virus in primary tissue, so that mutations associated with adaptation to cell culture are avoided. It is no more expensive than a neutralization assay. The only prerequisite for PCR is knowing the sequence of the regions flanking the portion of the genome to be detected, so that oligonucleotide primers that are complementary to a sequence on each strand of DNA can be made. PCR requires two primers, each of around 20–30 nucleotides in length, and these are chemically synthesized.

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