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By V.G. Berezkin, V.R. Alishoyev and I.B. Nemirovskaya (Eds.)

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Asymmetrical broadening of the peak of the main substance may be caused by various kinetic factors, such as slow kinetics of desorption of the substance from the sorbent grains or slow kinetics of desorption of the substance from stagnant zones. A theoretical explanation of the asymmetry of chromatographic zones due to kinetic effects in preparative separations was given by Giddings [42], and experimental verification was carried out in Takizawa et al. [43]. In selecting the optimal conditions for the separation of impurities and the main component, it is important to estimate the separation quantitatively in relation to the experimental parameters.

56 J . J. van Deemter, F. Zuiderweg and A. Klinkenbcrg, Cheni. Eng. S c i , S (1956) 271. 57 A. Klinkenherg and I:. Sjenitzer, Chem. Eng. , 5 (1956) 258. 58 J . C. Giddings, Dynamics of Chromatography, Part 1, Marcel Dekker, New York, 1965. 59 E. Glueckauf, in D. 11. Desty (Editor), Gas Orromatography 1958, Academic I’rcss, New York, 1958, p. 33. 60 G. Schay, Theoretische Gnindlageti der Gas-Chroniatoxraphie, VEB Deutscher Verlag der Wissenschaften, Berlin, 1960. 61 IUPAC, Pure Appl. , 37 (1974) 445.

0 0 0 .. .. .. .... .. 0 0 0 0 0 0 .. Pig. 10. Schematic representation of the chromatographic process based o n the theory of theoretical plates for compounds A (not retained by the stationary phase) and B (retained by the stationary phase). 0-4, successive stages of the chromatographic process. The plot depicts the distribution of concentrations of compounds A ( 0 ) and B ( 0 ) after five stages of gas phase transfer. Upper part of column, gas phase; lower part, liquid stationary phase.

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