By Professor Dr. Y. P. S. Bajaj (eds.)
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Although differences in rooting occurred among species and among cultivars within species, no specific relationship could be recognized. Shoots can also be rooted directly in soil or a soilless mix (Harris and Stevenson 1982; Chee et al. 1984), but the vines are very vulnerable to fungal infection at this stage. Alleweldt and Radler (1962) reported that a photoperiodic pretreatment of the mother plant affected subsequent growth activity in vitro in a genotype-dependent manner. Chee and Pool (1982) reported that a 10-h photoperiod was optimum for the establishment of Rougeon (Seibel 5898) cultures.
These authors reported that half-strength MS was superior to full strength MS, White's (1943) minerals, or White's minerals plus organics for rooting of axillary shoots. Leaf removal had a negative effect on rooting and best results were obtained with shoots 20- 25 mm long with three to five developed leaves. Vitamin D, ferrulic acid or charcoal, alone or with growth regulators, inhibited rooting. Morini et al. 5 and 1 mg/l lBA, respectively, for the five cultivars tested. They noted that the number of roots increased and their mean length decreased with increasing lBA concentration.
J Am Soc Hortic Sci 111 :634-638 Lebrun L (1985) Isolement et culture de protoplastes de vigne. In: Proc Moet-Hennessy ColI Amelioration de la vigne et culture in vitro, Paris, p 215 Lebrun L, Rajasekaran K, Mullins MG (1985) Selection in vitro for NaCI-tolerance in Vitis rupestris Scheele. Ann Bot (London) 56:733 - 739 Lee TC, Wicks T (1982) Dual culture of Plasmopara viticola and grapevine and its application to systemic fungicide evaluation. Plant Dis 66:308-310 Legin R, Bass P, Vuittenez A (1979) Premiers resultats de guerison par thermotherapie et culture in vitro d'une maladie de type cannelure (legno riccio) produite par Ie greffage du cultivar Servant de Vitis vinifera sur Ie porte-greffe Vitis riparia x V.