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By Mo Adam Mahmood, Gary J. Mann, Mark Dubrow

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Natl. Acad. Sci. , 99, 9942, 2002. 31. Calegari, F. , Tissue-specific RNA interference in postimplantation mouse embryos with endoribonuclease-prepared short interfering RNA, Proc. Natl. Acad. Sci. , 99, 14236, 2002. 32. , Gene synthesis machines: DNA chemistry and its uses, Science, 230, 281, 1985. pmd 34 7/26/2004, 10:57 AM 35 Basics of siRNA Design and Chemical Synthesis 33. Usman, N. , The automated chemical synthesis of long oligoribuncleotides using 2'O-silylated ribonucleoside 3'-O-phosphoramidites on a controlled-pore glass support: synthesis of a 43-nucleotide sequence similar to the 3'-half molecule of an Escherichia coli formylmethionine tRNA, J.

17 It will be important for the potential clinical use of this approach that an IGF1R inhibitor should block only its intended target, without influencing the expression, or function of the insulin receptor. This chapter describes the methods we used to identify regions of IGF1R mRNA that are most effectively targeted by RNA interference, and the implications of this information for the design of effective siRNAs. 18 In principle, this method maps the regions of the transcript that are accessible to binding with complementary oligonucleotides.

Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells, Proc. Natl. Acad. Sci. , 99, 9942, 2002. 31. Calegari, F. , Tissue-specific RNA interference in postimplantation mouse embryos with endoribonuclease-prepared short interfering RNA, Proc. Natl. Acad. Sci. , 99, 14236, 2002. 32. , Gene synthesis machines: DNA chemistry and its uses, Science, 230, 281, 1985. pmd 34 7/26/2004, 10:57 AM 35 Basics of siRNA Design and Chemical Synthesis 33.

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